General description

Native thrombospondin purified from human platelets. Adhesive glycoprotein released in response to platelet activation by α-thrombin. An angiogenesis inhibitor that blocks the FGF-induced neovascularization of the rat cornea. An inducer of platelet aggregation. Synthesized by a number of different cells and secreted into the extracellular matrix. Involved in the regulation of cellular proliferation.

Native thrombospondin purified from human platelets. Adhesive glycoprotein released in response to platelet activation by a-thrombin. Inhibits FGF-induced neovascularization on rat cornea. An inducer of platelet aggregation. Synthesized by a number of cells and secreted into the extracellular matrix. Involved in the regulation of cellular proliferation.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Armstrong, L.C., et al. 2002. Mol. Biol. Cell13, 1893.Michaund, M. and Poyet, O. 1994. Anticancer Res. 14, 1127.Castle, V.P., et al. 1993. J. Biol. Chem. 268, 2899.Koch, A.E., et al. 1993. Pathobiology 61, 1.Tolsma, S.S., et al. 1993. J. Cell Biol.122, 497.Tuszynski, G.P., et al. 1993. J. Cell Biol. 120, 513.Schon, P., et al. 1992. Eur. J. Cell Biol. 59, 329.Good, D.J., et al. 1990. Proc. Natl. Acad. Sci. USA87, 6624.

Packaging

25 µg in Plastic ampoule

Physical form

Lyophilized from 600 mM NaCl, 20 mM Tris, 1 mM CaCl₂, 20% sucrose, pH 7.8.

Preparation Note

Please refer to lot-specific reconstitution volume on the vial label.

Prepared from platelets that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for 1 week at 4°C or for at least 1 month at -70°C. Product is extremely adherent to surfaces; keep frozen (-70°C) before use and use siliconized surfaces to insure product recovery.

Warning

Toxicity: Standard Handling (A)